Journal: bioRxiv

Article Title: Monocytes Strongly Induce (MYO)Fibroblast Contraction in a New 3D Skin Model to Understand the Inflammation-Fibrosis Axis in Systemic Sclerosis

doi: 10.64898/2026.02.12.705496

Figure Lengend Snippet: ( A ) Representative immunohistochemistry staining showing clear CD68 expression in the monocyte-containing hydrogels (right). Black arrows indicate a “surrounding layer” formed by the CD68 + cells covering the fibroblasts. ( B ) Flow cytometry analyses showing the percentage of total macrophages (CD45 + CD68 + ) isolated from the hydrogels after enzymatic digestion and subsequently CD45 + magnetic selection. Cells (CD45 + CD68 + ) were classified into M1-like (CD163 − ) or M2-like (CD163 + ) macrophages. Comparisons were performed using Student’s t-test (*p<0.05). Each shape represents the average of 3 technical replicates of 1 PBMC donor, n = 3.

Article Snippet: Sections were then incubated with primary rabbit –anti-human alpha-smooth muscle actin (α-SMA) (1:200) (ab5694, Abcam, UK), – fibroblast activation protein (FAP) (1:100) (ab207178, Abcam), – Phospho-SMAD2 (1:100) (#3108L, CellSignaling, USA), – Phospho-Stat3 (1:50) (#9145, CellSignaling), or mouse anti-human – CD68 (#MCA1815, BioRad, USA) (1:200) antibodies, for 1 hour at room temperature.

Techniques: Immunohistochemistry, Staining, Expressing, Flow Cytometry, Isolation, Selection

Journal: Molecular Vision

Article Title: P2X 7 receptor activation may be involved in neuronal loss in the retinal ganglion cell layer after acute elevation of intraocular pressure in rats

doi:

Figure Lengend Snippet: Representative photomicrographs of double immunostaining of the P2X 7 receptor and CD68 in the normal retina and in the retina on days 1, 2, 3, and 7 after intraocular pressure elevation. P2X 7 -positive cells were also stained with anti-CD68 in the ganglion cell layer (GCL). Bar=100 µm.

Article Snippet: For this, mouse Alexa Fluor 488–labeled anti-TUJ1 monoclonal antibody (A488–435L, Covance Research Products, Princeton, NJ) and mouse anti-CD68 (MAB1435, Merck Millipore, Billerica, MA) were used.

Techniques: Double Immunostaining, Staining

Journal: Molecular Vision

Article Title: P2X 7 receptor activation may be involved in neuronal loss in the retinal ganglion cell layer after acute elevation of intraocular pressure in rats

doi:

Figure Lengend Snippet: Representative photomicrographs of double immunostaining of CD68 and TNF-α or interleukin-1β (IL-1 β) in the normal retina and the retina on day 2, with and without treatment of oxidized adenosine triphosphate (OxATP) at 30 µM just after intraocular pressure (IOP) elevation. A: TNF- α, B: IL-1 β. Immunoreactivities of tumor necrosis factor-α (TNF-α) and IL-1β were upregulated in the ganglion cell layer (GCL) and inner plexiform layer (IPL) cells on day 2 after IOP elevation; they were subsequently suppressed by treatment with OxATP. Bar=100 µm.

Article Snippet: For this, mouse Alexa Fluor 488–labeled anti-TUJ1 monoclonal antibody (A488–435L, Covance Research Products, Princeton, NJ) and mouse anti-CD68 (MAB1435, Merck Millipore, Billerica, MA) were used.

Techniques: Double Immunostaining

Journal: Clinics

Article Title: M2-Polarized tumor-associated macrophages are associated with poor prognoses resulting from accelerated lymphangiogenesis in lung adenocarcinoma

doi: 10.1590/S1807-59322011001100006

Figure Lengend Snippet: M2- and M1-polarized TAMs in lung adenocarcinoma. (A) The coexpression of CD68 and MMR in TAMs in human lung adenocarcinoma sections. (B) The coexpression of CD68 and iNOS in TAMs in human lung adenocarcinoma sections.

Article Snippet: Next, slides were immunostained using a monoclonal mouse anti-human CD68 antibody (1∶100; Zhongshan Company, Beijing, China).

Techniques:

Journal: BMC Cancer

Article Title: Local iron homeostasis in the breast ductal carcinoma microenvironment

doi: 10.1186/s12885-016-2228-y

Figure Lengend Snippet: FPN1-expressing leukocytes in carcinomas are predominantly M2-like. Sections of normal breast tissue, DCIS (ductal carcinoma in situ) and IDC (invasive ductal carcinoma) to reveal the presence of cells of the macrophage lineage (CD68), and its classical polarization phenotypes, M1-like (CD80) and M2-like (CD163), in FPN1-expressing leukocyte infiltrate. For details see Materials and Methods (Original magnification × 100- upper CD68 images, ×400- squared image series)

Article Snippet: Immunohistochemical staining was performed in 2 μm-thick TMA sections with the following antibodies: rabbit polyclonal anti-human hepcidin-25 antibody (dilution 1:500, Abcam, Cambridge, UK [ ]), rabbit polyclonal anti-human ferroportin 1 antibody (FPN—1:500, Novus Biologicals Europe, Cambridge, UK [ ]), rabbit polyclonal anti-human ferritin antibody (FT—1:1000, Sigma-Aldrich, MO, USA [ ]), mouse monoclonal anti-human CD71 (TFR1 [clone 10 F11]- 1:80, Novocastra, Newcastle, UK [ ]), mouse monoclonal anti-human CD68 (clone Kp-1, 1:2000, A. Menarini Diagnostics, CA, USA), mouse monoclonal anti-human CD163 (clone MRQ-26, 1:100, Cell Marque, CA, USA), mouse monoclonal anti-human CD80 (37711, 1:100, R&D Systems, MN, USA), rabbit polyclonal anti-human CD4 (clone H-370, 1:250, Santa Cruz Biotechnology, TX, USA) and mouse monoclonal anti-human CD8 (clone C8/144B, 1:100, Cell Marque, CA, USA).

Techniques: Expressing, In Situ

Journal: BMC Cancer

Article Title: Local iron homeostasis in the breast ductal carcinoma microenvironment

doi: 10.1186/s12885-016-2228-y

Figure Lengend Snippet: Representative images of the FPN1 analysis by Imaging Flow Cytometry in breast cancer core biopsies. Epithelial cells (EC) are stained by an anti-cytokeratin (CK) FITC. T lymphocytes (T Ly) were identifiable by CD3 PerCP-Cy5.5, B lymphocytes (B Ly) by CD20 PE-Cy7 and macrophages (M0) by CD68 PE-Cy7 (Original magnification × 400)

Article Snippet: Immunohistochemical staining was performed in 2 μm-thick TMA sections with the following antibodies: rabbit polyclonal anti-human hepcidin-25 antibody (dilution 1:500, Abcam, Cambridge, UK [ ]), rabbit polyclonal anti-human ferroportin 1 antibody (FPN—1:500, Novus Biologicals Europe, Cambridge, UK [ ]), rabbit polyclonal anti-human ferritin antibody (FT—1:1000, Sigma-Aldrich, MO, USA [ ]), mouse monoclonal anti-human CD71 (TFR1 [clone 10 F11]- 1:80, Novocastra, Newcastle, UK [ ]), mouse monoclonal anti-human CD68 (clone Kp-1, 1:2000, A. Menarini Diagnostics, CA, USA), mouse monoclonal anti-human CD163 (clone MRQ-26, 1:100, Cell Marque, CA, USA), mouse monoclonal anti-human CD80 (37711, 1:100, R&D Systems, MN, USA), rabbit polyclonal anti-human CD4 (clone H-370, 1:250, Santa Cruz Biotechnology, TX, USA) and mouse monoclonal anti-human CD8 (clone C8/144B, 1:100, Cell Marque, CA, USA).

Techniques: Imaging, Flow Cytometry, Staining